Chemistry and Toxicology of Atmospheric Emissions Team (CTEA)

Research activity

Our research focuses on assessing exposure levels to gaseous and particulate air pollutants in suspension in the air (PM10, PM2.5, ultrafine) and studying their toxic effects in humans, particularly in the respiratory system.

The development of this theme is based on strategies for sampling particles suspended in outdoor air, their physicochemical characterisation and the evaluation of their toxicity using experimental models (in vitro, ex vivo, in vivo) or carried out in humans. The aim is to examine the underlying physiopathological mechanisms frequently suspected of being involved in the development of respiratory pathologies, and to propose biomarkers of exposure or the effect of atmospheric pollution.

Our approach is based on two main orientations:

Physicochemistry of suspended particles (PM10, PM2.5, ultrafine) and identification of sources

Particulate sampling is carried out in urban or rural areas, subject to various natural or anthropogenic influences such as road traffic, industrial activities or marine emissions. The aim of examining the chemical characteristics of particles (ionic species, major elements and metallic traces, organic compounds) is to identify and/or propose source tracers to enable the origin of particles to be determined. Source-receptor models are used to identify sources and assess their contributions to PM concentration in the atmosphere.

Mechanisms of toxic action of air pollutants

Based on experimental toxicology, we are helping to improve knowledge of changes in cellular homeostasis induced by exposure to environmental pollutants by characterizing oxidant stress parameters, inflammatory response, metabolic activation, genotoxicity and epigenetic alterations. The evaluation of these toxic effects is carried out in-vitro on pulmonary and blood cell line models (A 459, BEAS-2B, THP-1) in mono and/or co-culture but also on primary cells (alveolar macrophages, lymphocytes, blood monocytes) in order to better integrate the cellular diversity of the respiratory tract. The study of these mechanisms of toxic action, using molecular, cellular and metabolomic biology methods, makes it possible to propose new biological markers of exposure or effect potentially involved in the effects of environmental pollutants on human health.

Team members

BILLET SylvainMCF-HDR
BOUHSINA SaadMCF
COURCOT DominiquePr
GOSSET PierreEC GHICL
LANDKOCZ YannMCF
LEDOUX FrédéricPr
MARTIN PerrineMCF
TREMOLET GauthierTech Contractuel
VERDIN AnthonyIR

Scientific collaborations

 Nationals :

  •  Unité Aliment Bioprocédés Toxicologie Environnements, ABTE, EA 4651, Equipe « Toxicologie de l’Environnement : milieux aériens et cancers, ToxEMAC », Université de Caen Basse Normandie, Université de Rouen
  • Groupement des Hôpitaux de l’Institut Catholique de Lille, GHICL, Service de Pneumologie, Service de Médecine et Gériatrie
  • Laboratoire Impact de l’Environnement Chimique sur la Santé Humaine, Impecs, EA 4483, Université de Lille
  • Laboratoire Lésions des Acides Nucléiques, Service de Chimie Inorganique et Biologique, UMR E3 – CEA / Université Joseph Fourier, Grenoble
  • Institut de Recherches en Santé-Environnement-Travail – IRSET – UMR Inserm 1085, Faculté des Sciences pharmaceutiques et biologiques de Rennes, Université de Rennes
  • Institut National de l’Environnement Industriel et des Risques, INERIS, Verneuil en Halatte
  • Laboratoire Génie Civil et géoEnvironnement, LGCgE, EA 4515, groupe ISA de Lille
  • IMT Lille-Douai, Département Sciences de l’Atmosphère Génie de l’Environnement, SAGE.
  • Laboratoire de Santé Publique et Environnement, EA 4064 « Impacts sanitaires des pollutions », Université Paris Descartes

 Internationals :

  •  Departemento de Ingenierias Quimica y Biomolecular, Universidad de Cantabria, Santander, Espagne.
  • Faculté de Médecine, Université de Cagliari, Italie.
  • Bioactive Molecules Research Group, Department of Chemistry and Biochemistry, Faculty of Sciences-2, Lebanese University, Liban.
  • Department of Biology, Faculty of Sciences, University of Balamand, Liban.
  • King’s College London, Environmental Research Group, Royaume-Uni.
  • Equipe  » Thermodynamique et Génie des Procédés Chimiques « , Département Chimie, Faculté Polytechnique de l’Université de Mons, Belgique.
  • Laboratoire de Toxicologie, Faculté de Médecine Pharmacologie Odontologie, Université Cheikh Anta Diop de Dakar, Sénégal.
  • Norwegian Institute of Public Health, Department of Chemical and Radiation, Norway
  • Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Bologna, Italy
  • Shandong University, Environment Research Institute, China

current thesis

Name

Period

Title

Méausoone Clémence

2016-2019

Human health impact of air pollutants: assessment of toxicity of emissions from catalytic oxidation of industrial VOCs
Badran Ghidaa

2016-2019

Toxic effects of PM2.5 Polycyclic Aromatic Hydrocarbons (PAHs) on bronchial epithelial cells
Junmei Zhang

2016-2019

PM2.5 in Northern China: concentration, composition, source apportionment and toxicity assessment
Lamia Mouffarej

2017-2020

Study of the physicochemical characteristics and determinants of pulmonary toxicity of fine and ultrafine particles
Stéphanie Al Koussa

2018-2021

Conséquences de l’exposition aux PM2.5 sur les vésicules extracellulaires (VE) produites par les cellules épithéliales pulmonaires : profil en miARN et effets sur le microenvironnement immunitaire
Marc Fadel

2018-2021

Caractérisation des aérosols et de leurs sources dans des milieux industriels au Liban

Research agreement

  • 2015-2019 : VESITOX Project “Extracellular vesicles as potential new biomarkers of toxicity of environmental pollutants” – ANSES Environment and Health APR, funded by INSERM and Aviesan
  • 2016-2020 : Interreg V France Wallonia Flanders project “DepollutAir : Innovative Couplings of Volatile Organic Compounds (VOC) treatment techniques: new ways to improve air quality”.
  • 2016-2019 : TRIPLER project ” Impact of PM2.5 varying according to their source (road traffic, industries) on respiratory health. Approche in vitro ” – APR Environnement-Santé de l’ANSES.
  • 2015-2020 : CPER Climibio Project, WP3, “WP3: Impact of climate change and environmental dynamics on living organisms”.
  • 2018-2022 : Projet BIONEUROMET « Environmental exposure to particulate matter-bound manganese and other metals: bioaccessibility, biomonitoring, and their association with the neurological function”, Spanish Research Agency, Project leader : Universidad de Cantabria, Santander, Espagne

Recent publications

  • A. NDONG, F. CAZIER, A. VERDIN, G. GARÇON, M. CABRAL, L. COURCOT, A. DIOUF, M. GUALTIERI, D. COURCOT, M. FALL (2019). Physico-chemical characterization and in vitro inflammatory and oxidative potency of atmospheric particles collected in Dakar city (Senegal). Environmental Pollution, 245, 568-581. (doi:10.1016/j.envpol.2018.11.026)
  • T. DOUKI, C. CORBIERE, D. PRETERRE, P.J. MARTIN, V. LECUREUR, V. ANDRE, Y. LANDKOCZ, I. POTTIER, V. KERAVEC, O. FARDEL, S. MOREIRA-REBELO, D. POTTIER, C. VENDEVILLE, F. DIONNET, P. GOSSET, S. BILLET, C. MONTEIL, F. SICHEL (2018). Comparative study of diesel and biodiesel exhausts on lung oxidative stress and genotoxicity in rats. Environ Pollut. 235, 514-524. (https://doi.org/10.1016/j.envpol.2017.12.077)
  • F.LEDOUX, C.ROCHE, F.CAZIER, C. BEAUGARD, D.COURCOT (2018). Influence of ship emissions on NOx, SO2, O3 and PM concentrations in a North-Sea harbour in France. J. Environ. Sci., 71, 56-66. (doi.org/10.1016/j.jes.2018.03.030)
  • S. BILLET, Y. LANDKOCZ, P. MARTIN, A. VERDIN, F.LEDOUX, C.LEPERS, V. ANDRE, F.CAZIER, F.SICHEL, P.SHIRALI, P.GOSSET, D.COURCOT (2018). Chemical characterization of fine and ultrafine PM, direct and indirect genotoxicity of PM and their organic extracts on pulmonary cells. J. Environ. Sci., 71, 168-178. (doi.org/10.1016/j.jes.2018.04.022)
  • B. FOUGÈRE, Y. LANDKOCZ, C. LEPERS, P.J. MARTIN, L. ARMAND, N. GROSSIN , A. VERDIN, E. BOULANGER, P. GOSSET, F. SICHEL, P. SHIRALI, S. BILLET (2018), Influence of aging in the modulation of epigenetic biomarkers of carcinogenesis after exposure to air pollution, Experimental Gerontology, 110, 125-132. (doi.org/10.1016/j.exger.2018.05.018)
  • I. ABBAS, G. BADRAN, A. VERDIN, F. LEDOUX, M. ROUMIÉ, D. COURCOT, G. GARÇON (2018). Polycyclic aromatic hydrocarbon derivatives in airborne particulate matter: sources, analysis and toxicity. Environ. Chem. Lett. 16, 439-475 (DOI: 10.1007/s10311-017-0697-0)
  • A. HÉLIOT, Y. LANDKOCZ, F. ROY SAINT-GEORGES, P. GOSSET, S. BILLET, P. SHIRALI, D. COURCOT, P.J. MARTIN (2017). Smoker extracellular vesicles influence status of human bronchial epithelial cells. Int. J. Hyg. Environ. Health. 220 (2 Pt B) : 445-454.
  • Y. LANDKOCZ, F. LEDOUX, V. ANDRÉ, F. CAZIER, P. GENEVRAY, D. DEWAELE, P.J. MARTIN, A. VERDIN, L. ARMAND, C. LEPERS, L. COURCOT, S. BOUHSINA, F. SICHEL, P. SHIRALI, M. GUALTIERI, D. COURCOT, S. BILLET (2017) Fine and ultrafine atmospheric Particulate Matter at a multi-influenced urban site: Physicochemical Characterization, Mutagenicity and Cytotoxicity. Environ Pollut. 221, 130-140.
  • A. HERNANDEZ-PELLON, I. FERNANDEZ-OLMO, F. LEDOUX, L. COURCOT, D. COURCOT (2017) Characterization of manganese-bearing particles in the vicinities of a manganese alloy plant. Chemosphere, 175, 411-424.
  • F. LEDOUX, A. KFOURY, G. DELMAIRE, G. ROUSSEL, A. EL ZEIN, D. COURCOT (2017) Contributions of local and regional anthropogenic sources of metals in PM2.5 at an urban site in northern France. Chemosphere, 181, 713-724.
  • P.N. MELKI, F. LEDOUX, S. AOUAD, S. BILLET, B. EL KHOURY, Y. LANDKOCZ, R.M. ABDEL-MASSIH, D. COURCOT (2017) Physicochemical characteristics, mutagenicity, and genotoxicity of airborne particles under industrial and rural influences in Northern Lebanon. Environmental Science and Pollution Research, 24, 18782-18797.
  • M. AL ZALLOUHA, Y. LANDKOCZ, BRUNET J., COUSIN R., GENTY E., D. COURCOT, SIFFERT S., P. SHIRALI, S. BILLET (2017) Usefulness of toxicological validation of VOCs catalytic degradation by air-liquid interface exposure system. Environ. Res. 152, 328–335.