Unité de Chimie Environnementale et Interactions sur le vivant

Plant-Fungus Interactions and Remediation Team (IPCR)

Team Leader : Anissa LOUNES-HADJ SAHRAOUI

Team presentation :

Research activity

The IPCR team’s research activities are based on its expertise in mycology, in particular on the interactions between plants and fungi (phytopathogenic as well as symbiotic fungi), and in physiology, cytology, biochemistry, molecular biology and microbial ecology. They are organized in two main fields :

1 – Biocontrol of wheat foliar fungal diseases

This research theme aims to develop new biocontrol and sustainable control strategies against wheat fungal parasites, using Plant Defense Stimulants (PDSs) and/or beneficial bacterial (Plant Growth-Promoting Rhizobacteria) or fungal (Plant Growth-Promoting Fungi) microorganisms such as arbuscular mycorrhizal fungi and/or biostimulants in plant protection. Our studies focus on two pathosystems in particular, wheat/Blumeria graminis f.sp. tritici (agent of powdery mildew) and wheat/Zymoseptoria tritici (agent of septoria). The study of defense reactions in wheat and the optimization of the protective efficacy of SDPs in response to various environmental factors that may influence the establishment of such protection (wheat genotype, nutritional status of the plant, soil microbiota, climate change, etc.) are the main objectives of our work.

2- Phytomanagement and Bioremediation of contaminated soils.

This research theme aims to develop alternative, gentle and sustainable methods of remediation and management of contaminated soils.

In the case of phytomanagement, the feasibility, effectiveness and sustainability of the main phytotechnologies (phytostabilisation, phyto/rhizodegradation and phytoextraction) are being studied from the laboratory scale under controlled conditions to that of in situ workshop sites (demonstrators).
The aim is (1) to study the performance of different plant species and soil improvers, especially biological ones based on arbuscular mycorrhizal fungi, in the fate (mobility, transfer, dissipation) of organic pollutants (e.g., in the form of a fungus): PAHs, dioxins/furans, alkanes) and inorganic pollutants (trace elements), (2) to develop non-food channels for the valorisation of phytomass produced on polluted soils, (3) to monitor the influence of phytotechnologies on the refunctioning of contaminated soils, in particular via the characterisation of their microbiota and (4) to assess the environmental and health risks after treatment.

In a complementary way, bioremediation consists in exploiting, in particular, the diversity of saprotrophic fungi within the framework of the biodegradation of organic pollutants such as PAHs, dioxins/furans and emerging pollutants not only in the soil but also in other matrices (water, sediments, gaseous effluents, biogas). This work aims to study the mechanisms involved in the degradation of organic pollutants (availability, transport and incorporation, degradation), and the interest of using natural polysaccharides (modified starches) as solubilising agents for these pollutants.

Team members

PERMANENT STAFF

 

BOURDON-FACON Natacha

TECH

FONTAINE Joël    

Pr

GRANDMOUGIN-FERJANI Anne

PR

LARUELLE Frédéric

IR

LOUNES-HADJ SAHRAOUI  Anissa

PR

MAGNIN-ROBERT Maryline

MCF

RAFIN Catherine

MCF-HDR

RANDOUX Béatrice

MCF

TISSERANT Benoît

IR

VEIGNIE Etienne

MCF

NON-PERMANENT STAFF

 

ALLARIO Thierry

Post-Doc 2022

ISRAEL Abir

ATER 2022

Scientific collaboration

 Régionals :

  • BioGAP, Institut Charles Viollette – ISA de Lille
  • ProBIOGEM, Institut Charles Viollette –Université de Lille
  • Laboratoire  Génie  Civil  et  géoEnvironnement, Université de Lille

Nationals :

  •  Unité de Recherche Vigne et Vin de Champagne, URCA à Reims
  • UMR Agroécologie, INRA de Dijon
  • Arvalis-Institut du Végétal

 Internationals :

  •  Laboratoire d’Amélioration Génétique des Plantes, Université d’Annaba (Algérie)
  • Université des Sciences et de la Technologie Houari Boumédiène (USTHB, Algérie)
  • Université de Djelfa
  • Helmholtz Zentrum München – German Research Centre for Environmental Health, Institute of Soil Ecology, Allemagne
  • Laboratory of Phytopathology, Université de Gand (Belgique)
  • Unité de Chimie Biologique Industrielle, Université de Liège (Gembloux Agro Bio Tech) (Belgique)
  • Laboratoire de Mycologie, Université de Louvain-la-Neuve (Belgique)
  • Agriculture et Agroalimentaire (Canada)
  • Institute for Biological Sciences (Canada)
  • Université de Wisconsin-Madison (USA)
  • Crop & Soil Systems Research Group, Scottish Agricultural College (Grande-Bretagne)
  • Faculté d’Agronomie, Université Saint-Esprit de Kaslik, université Libanaise (Liban)
  • Université Vasile Alecsandri de Bacau, Roumanie
  • Laboratoire de Génétique et Amélioration des Plantes, INAT de Tunis, Centre National de la Recherche en Agriculture Oasienne (Tunisie)
  • Centre National de la Recherche en Agriculture Oasienne (Tunisie)

Current Thesis

Prénom Nom

Période

Titre

Amandine Ducousso2018-2021Influence of plant-microbe-mycorrhizal interaction on the biological dissolution of phosphates
Julien Langrand2020-2023Phytomanagement for the refunctionalization of polluted soils and ecosystem services: optimizing the essential oil sector in the bioeconomy
Oumaima Akachoud2020-2023Contribution of mycorrhizal inoculation in the production of essential oils in some MAPs of the Marrakech region
Mathieu Delaeter2021-2024Development of specific molecular markers for resistance induction in wheat in response to mycorrhizal inoculation to control two fungal diseases: septoria and powdery mildew
Camille Carton2021-2024PROposition of new BIOsourced molecules of plant origin in the fight against plant diseases

Research agreement

Internationals Projects

  • 2021-2024 : PHC-TOUBKAL project : Contribution of mycorrhizal inoculation in the production of essential oils in some MAPs of the Marrakech region, in collaboration with a Moroccan partner: A. Qaddoury, Cadi Ayyad University.
  • 2016-2021 : INTERREG V France-Flanders-Walloon project, ” Project portfolio : SMARTBIOCONTROL (26 partners from university laboratories, research centres or farmers’ unions).
    • BIOSCREEN project: “New biosourced and multifunctional molecules for the control of phytopathogenic agents in crops in the cross-border region”. Some of them will be derived from CMA.
    • BIOPROTECT project: “Biological protection in practice: optimisation of the effectiveness of (new) biological pesticides in the field”. Different CMA species will be tested for their protective potential against powdery mildew and septoria in wheat.

Nationals Projects

  • 2021-2024 : PIA – Programme d’Investissement d’Avenir – Ville Durable – Site du Crouy : Applied scientific and technical research project aimed at evaluating the relevance of using locally produced composts in the sustainable management of degraded soils in dense urban areas (5 partners).
  • 2021-2024 :  DEPHYTOP project (ADEME, APR GRAINE): Demonstrator of phytomanagement of soils contaminated by TMEs based on the circular economy: Optimisation of the essential oils sector (10 academic, private and institutional partners).
  • 2017-2021 : PHYTEO project, Phytostabilization and Essential Oil (ADEME, APR GRAINE): Essential oil production: an eco-innovative pathway for the conversion of historically polluted soils (6 academic and private partners).
  • 2018-2022 : EXTRA-Zn project (ADEME, APR GRAINE): ): Production of Zn-enriched biomasses by phytoextraction for ecocatalysis (4 partners).

Regionals Projects

  • CPER BiHauts-Eco de France
  • CPER ECRIN
  • 2022-2025 : PROZYMO project (Stimule project): Enzymatic production of new plant defence molecules.
  • 2016 – 2022 : CPER  project « ALIBIOTECH Food and Biotechnology ».
    WP 1. Characterization of the plant’s interaction with its environment
    WP 2. Biological activities of biomolecules and secondary metabolites from plants and/or micro-organisms
  • 2019 – 2021 : A2U project : ULCO/UPJV. Tripartite interaction as an alternative control method against powdery mildew and septoria in wheat, TRIPLET.
  • 2022: VANIFUN project (SFR Condorcet): Evaluation of vanillin derivatives: application in plant protection against fungal pathogens.
  • 2021 : PHYTOFONC project (SFR Condorcet): Contribution of organic amendments in the phytostabilisation of metallic trace elements and the refunctionalisation of the soil.

Private research contracts

  • STOLZ industrial contract (2017, 2018 then 2020): Monitoring the microbiological quality (fungi and bacteria) of animal meal during an innovative sterilisation process.
  • LHOIST industrial contract (2019): Study of the antifungal properties, in vitro, of 2 products (Ca(OH)2 and CaCO3) on 3 phytopathogenic fungi of wheat.

Recent publications

  • Ducousso-Détrez, A.; Fontaine, J.; Lounès-Hadj Sahraoui, A.; Hijri, M. Diversity of Phosphate Chemical Forms in Soils and Their Contributions on Soil Microbial Community Structure Changes. Microorganisms, 2022, 10, 609. https://doi.org/10.3390/microorganisms10030609.
  • RAVEAU, R.; FONTAINE, J.; SOLTANI, A.; MEDIOUNI BEN JEMÂA, J.; LARUELLE, F.; LOUNÈS-HADJ SAHRAOUI, A. : In Vitro Potential of Clary Sage and Coriander Essential Oils as Crop Protection and Post-Harvest Decay Control Products. Foods 2022, 11, 312, 2022. https://doi.org/10.3390/ foods11030312.
  • M.C. de BORBA, A.C. VELHO, M.B. de FREITAS, M. HOLVOET, A. MAIA-GRONDARD, R. BALTENWECK, M. MAGNIN-ROBERT, B. RANDOUX, J.-L. HILBERT, Ph REIGNAULT, P. HUGUENEY, A. SIAH, M.J. STADNICK. A laminarin-based formulation protects wheat against Zymoseptoria tritici via direct antifungal activity and elicitation of host defense-related genes. Plant Disease. First Look 2022.
  • M. JERBI, S. LABIDI, F. LARUELLE, B. TISSERANT, F. BEN JEDDI, A. LOUNÈS-HADJ SAHRAOUI. : Mycorrhizal biofertilization improves grain yield and quality of Hulless Barley (Hordeum vulgare ssp. nudum L.) under water stress conditions. Journal of Cereal Science, 104, 2022. doi:https://doi.org/10.1016/j.jcs.2022.103436.
  • M. JERBI, S. LABIDI, F. LARUELLE, B. TISSERANT, Y. DALPE, A. LOUNES-HADJ SAHRAOUI, F. BEN JEDDI. : Contribution of native and exotic arbuscular mycorrhizal fungi in improving the physiological and biochemical response of Hulless Barley (Hordeum vulgare ssp. nudum L.) to drought. Journal of Soil Science and Plant Nutrition, 2022. DOI:10.1007/s42729-022-00802-2.
  • A. LOUNES-HADJ SAHRAOUI, M. CALONNE-SALMON, S. LABIDI, H. MEGLOULI, J. FONTAINE  ; Chapter 3 : Arbuscular mycorrhizal fungi-assisted phytoremediation: Concepts, challenges, and future perspectives. In : Assisted PhytoremediationEdited by V. Pandey. Elsevier. 2022. Pp49-100. https://doi.org/10.1016/C2019-0-04894-7.
  • BENCHERIF K, LARUELLE F, DALPÉ Y, LOUNÈS-HADJ SAHRAOUI A. : Inoculum Sources Modulate Mycorrhizal Inoculation Effect on Tamarix articulata Development and Its Associated Rhizosphere Microbiota. Plants. 2021; 10(12):2716. https://doi.org/10.3390/plants10122716.
  • A. GRIGNET, A. LOUNÈS-HADJ SAHRAOUI, S.TEILLAUD, J.FONTAINE, A. PAPIN, V. BERT. : Phytoextraction of Zn and Cd with Arabidopsis halleri: a focus on fertilization and biological amendment as a means of increasing biomass and Cd and Zn concentrations. Environ Sci Pollut Res (2021). https://doi.org/10.1007/s11356-021-17256-1.
  • RAVEAU R, FONTAINE J, VERDIN A, MISTRULLI L, LARUELLE F, FOURMENTIN S, LOUNÈS-HADJ SAHRAOUI A. : Chemical Composition, Antioxidant and Anti-Inflammatory Activities of Clary Sage and Coriander Essential Oils Produced on Polluted and Amended Soils-Phytomanagement Approach. Molecules, 2021, 26, 5321. https://doi.org/10.3390/molecules26175321.
  • R. RAVEAU, FONTAINE, J., BERT, V., PERLEIN, A., TISSERANT, B., FERRANT, P., LOUNES-HADJ SAHRAOUI, A. : In situ cultivation of aromatic plant species for the phytomanagement of an aged-trace element polluted soil: plant biomass improvement options and techno-economic assessment of the essential oil production channel. Sci. Total Environ. 147944, 2021. doi:10.1016/j.scitotenv.2021.147944.
  • RAVEAU, R., LOUNES-HADJ SAHRAOUI, A., HIJRI, M., FONTAINE, J. : Clary sage cultivation and mycorrhizal inoculation influence the rhizosphere fungal community of an aged trace-element polluted soil. Microorganisms 9, 1333, 2021. doi:10.3390/microorganisms9061333.
  • PERLEIN, A., ZDANEVITCH, I., GAUCHER, R., ROBINSON, B., PAPIN, A., LOUNES-HADJ SAHRAOUI, A., BERT, V. : Phytomanagement of a metal(loid)-contaminated agricultural site using aromatic and medicinal plants to produce essential oils: analysis of the metal(loid) fate in the value chain. Environmental Science and Pollution Research, 2021. doi.org/10.1007/s11356-021-15045-4.
  • JERBI M., S. LABIDI, B. A. BAHRI, F. LARUELLE, B. TISSERANT, F. BEN JEDDI, A. LOUNÈS-HADJ SAHRAOUI : Soil properties and climate affect arbuscular mycorrhizal fungi and soil microbial communities in Mediterranean rainfed cereal cropping systems, Pedobiologia, Volumes 87–88, 2021. doi.org/10.1016/j.pedobi.2021.150748.
  • HONVAULT N., D. HOUBEN, S. FIRMIN, H. MEGLOULI, F. LARUELLE, J. FONTAINE, A. LOUNÈS-HADJ SAHRAOUI, A. COUTU, H. LAMBERS, M-P FAUCON, : Interactions between below-ground traits and rhizosheath fungal and bacterial communities for phosphorus acquisition. Funct Ecol. 2021; 00: 1-17. doi.org/10.1111/1365-2435.13823.
  • R. PLATEL, M. SAWICKI, Q. ESMAEL, B. RANDOUX, P. TRAPET, M. EL GUILLI, N. CHTAINA, S. ARNAULD, A. BRICOUT, A. ROCHEX, N. BOURDON, P. HALAMA, C. JACQUARD, E. AIT BARKA, Ph. REIGNAULT, M. MAGNIN-ROBERT, A. SIAH. Isolation and identification of lipopeptide-producing Bacillus velezensis strains from wheat phyllosphere with antifungal activity against the wheat pathogen Zymoseptoria tritici. Agronomy. 12 (1) : 95, 2021. doi.org/10.3390/agronomy12010095 2021.
  • M.C. DE BORBA, A.C. VELHO, A. MAIA-GRONDARD, R. BALTENWECK, M. MAGNIN-ROBERT, B. RANDOUX, M. HOLVOET, J.-L. HILBERT, C. FLAHAUT, Ph. REIGNAULT, P. HUGUENEY, M.J. STADNIK, A. SIAH. The algal polysaccharide ulvan induces resistance in wheat against Zymoseptoria tritici without major alteration of leaf metabolome. Front. Plant Sci. 2021. https://doi.org/10.3389/fpls.2021.703712.
  • R. PLATEL, L. CHAVERIAT, S. LE GUENIC, R. PIPELEERS, M. MAGNIN-ROBERT, B. RANDOUX, P. TRAPET, V. LEQUART, N. JOLY, P. HALAMA, P. MARTIN, M. HOFTE, Ph REIGNAULT, A. SIAH : Importance of the C12 carbon chain in the biological activity of rhamnolipids conferring protection in wheat against Zymoseptoria tritici. Molecules 26:40, 2021. https://doi.org/10.3390/molecules26010040.
  • BENCHERIF K., TRODI F., HAMIDI M., DALPÈ Y., LOUNÈS – HADJ SAHRAOUI A. : Biological Overview and Adaptability Strategies of Tamarix Plants, T. articulata and T. gallica to Abiotic Stress. In: Giri B., Sharma M.P. (eds) Plant Stress Biology. Springer, Singapore, 2021. https://doi.org/10.1007/978-981-15-9380-2_14.
  • KRZYZANIAK Y., MAGNIN-ROBERT M., RANDOUX B., FONTAINE J., LOUNÈS-HADJ SAHRAOUI A. : Combined Use of Beneficial Bacteria and Arbuscular Mycorrhizal Fungi for the Biocontrol of Plant Cryptogamic Diseases: Evidence, Methodology, and Limits. In: Shrivastava N., Mahajan S., Varma A. (eds) Symbiotic Soil Microorganisms. Soil Biology, vol 60, 2021. Springer, Cham. https://doi.org/10.1007/978-3-030-51916-2_24.
  • S. MORVAN, H. MEGLOULI, A. LOUNÈS-HADJ SAHRAOUI, M. HIJRI. : Into the wild blueberry ( Vaccinium angustifolium ) rhizosphere microbiota. Environmental Microbiology Volume 22, Issue 9 p. 3803-3822, 2020. https://doi.org/10.1111/1462-2920.15151.
  • R. RAVEAU, J. FONTAINE, M. HIJRI AND A. LOUNES-HADJ SAHRAOUI : The culture of clary sage shaped the rhizospheric bacterial communities more strongly than mycorrhizal inoculation in the trace element-contaminated soil -a two-year monitoring field trial. Frontiers in Microbiology, 2020. https://doi.org/10.3389/fmicb.2020.586050.
  • JERBI M, LABIDI S, LOUNÈS-HADJ SAHRAOUI, A, CHAAR H, BEN JEDDI F : Higher temperatures and lower annual rainfall do not restrict, directly or indirectly, the mycorrhizal colonization of barley (Hordeum vulgare L.) under rainfed conditions. PLoS ONE 15(11): e0241794, 2020. https://doi.org/10.1371/journal.pone.0241794.
  • H. MEGLOULI, J. FONTAINE, A. LOUNES-HADJ SAHRAOUI. : Dioxins/furans disturb the life cycle of an arbuscular mycorrhizal fungus, Rhizophagus irregularis, grown in vitro”. International Journal of Phytoremediation, 2020, 22(14):1497-1504. DOI: 10.1080/15226514.2020.1784089.
  • M. PIERISTE, E. FOREY, A. LOUNES-HADJ SAHRAOUI, H. MEGLOULI, F.LARUELLE, P. DELPORTE, T M. ROBSON, M. CHAUVAT. : Spectral composition of sunlight affects the microbial functional structure of beech leaf litter. Plant and Soil, 451, 515-530, 2020. DOI: 10.1007/s11104-020-04557-6.
  • A. YACOUMAS, N. HONVAULT & D. HOUBEN, J. FONTAINE, H. MEGLOULI, F. LARUELLE, B. TISSERANT, M.-P. FAUCON, A. LOUNES-HADJ SAHRAOUI, S. FIRMIN. : Contrasting Response of Nutrient Acquisition Traits in Wheat Grown on Bisphenol A-Contaminated Soils. Water Air Soil Pollut 231:23, 2020. DOI: 10.1007/s11270-019-4383-7.
  • I. EL-ALAM, R. RAVEAU, J. FONTAINE, A. VERDIN, F. LARUELLE, S. FOURMENTIN, R. CHAHINE, H. MAKHLOUF, A. LOUNES-HADJ SAHRAOUI : Antifungal and Phytotoxic Activities of Essential Oils: In Vitro Assays and Their Potential Use in Crop Protection. Agronomy 10, 825, 2020. https://doi.org/10.3390/agronomy10060825.
  • M. JERBI, S. LABIDI, A. LOUNES-HADJ SAHRAOUI, Y. DALPE, F. BEN JEDDI : Native arbuscular mycorrhizal fungi enhance plant growth and productivity of hulless barley (Hordeum vulgare ssp nudum L.). Journal of new sciences, Agriculture and Biotechnology, 78 (3), 4560-4569, 2020. www.jnsciences.org, E-ISSN 2286-5314.
  • L. SOMAI-JEMMALI, A. SIAH, B. RANDOUX, M. MAGNIN-ROBERT, P. HALAMA, W. HAMADA, Ph. REIGNAULT : Brown alga Ascophyllum nodosum extract-based product, Dalgin Active®, triggers defense mechanisms and confers protection in both bread and durum wheat against Zymoseptoria tritici. J. Appl. Phyco., 32, 3387-3399, 2020. DOI: 10.1007/s10811-020-02224-y.
  • S. MEJRI, M. MAGNIN-ROBERT, B. RANDOUX, A. GHINET, P. HALAMA, A. SIAH, Ph. REIGNAULT : Saccharin provides protection and activates defense mechanisms in wheat against the hemibiotrophic pathogen Zymoseptoria tritici. Plant Dis. 105, 780-786, 2020. https://doi.org/10.1094/PDIS-05-20-1106-RE.
  • F. STEFANI, K. BENCHERIF , S. SABOURIN, A. LOUNES HADJ-SAHRAOUI, C. BANCHINI, S. SÉGUIN, Y. DALPÉ : Taxonomic assignment of arbuscular mycorrhizal fungi in 18S metagenomic dataset: a case study with the saltcedar (Tamarix aphylla). Mycorrhiza, 2020.  https://doi.org/10.1007/s00572-020-00946-y.
  • H. HADJ YOUCEF TAIBI, N. SMAIL-SAADOUN, . LABIDI, K. ABDELLAOUI, M. MAKHLOUF, A. LAOUAR, C. TAGMOUNT BENOUARET, L. REZKI-SEKHI, A. BELKEBIR BOUKAIS,, A. LOUNES-HADJ SAHRAOUI : The Influence of No-till Farming on Durum Wheat Mycorrhization in a Semi-Arid Region: A Long-Term Field Experiment. Journal of Agricultural Science Vol. 12, No. 4 77-96, 2020. DOI:10.5539/jas.v12n4p77.
  • R. RAVEAU, J. FONTAINE, A. LOUNÈS-HADJ SAHRAOUI : Essential Oils as Potential Alternative Biocontrol Products against Plant Pathogens and Weeds: A Review. Foods2020, 9(3), 365, 2020. https://doi.org/10.3390/foods9030365.
  • A. GRIGNET, A. DEVAUFLEURY, A. PAPIN, V. BERT : Urban soil phytomanagement for Zn and Cd in situ removal, greening, and Zn-rich biomass production taking care of snail exposure. Environ. Sci. Pollu. Res., 27(3), 3187-3201 , 2020. doi: 10.1007/s11356-019-06796-2.